Overall, the current study carried out on the fatty acids composition of meat of local poultry population of Gallus gallus species of Benin reared under free range and conventional breeding systems is the first one on the characterization of these local poultry populations. Overall, palmitic and oleic acids were the most abundant fatty acids in the various meats under analysis. Нe highest SFA, MUFA and PUFA concentrations were found respectively in Holli, Fulani and North ecotypes.